In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Shimadzu 2450 UV–Vis spectrophotometer and SpectraMax M5 microplate reader were used to perform the optical characterization and measurements. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 04 to 3. 312 gain calibration failed. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Call 516-939-0888 to check. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The following document is intended to demonstrate setup of this instrument. Fluorescence Microplate Readers. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideFluorescence Microplate Reader Comparison Chart. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. Table of Contents. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. When a. SoftMax Pro. This. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. All reagents were brought to room temperature before use. M3-M5e_manual. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 0 . Parallel negative control assays had primary, secondary, or both antibodies omitted. Supersedes 1. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The spectrophotometric. We would like to show you a description here but the site won’t allow us. Microplate Readers are modular and . Add to Cart. Add to Cart. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 7°C (gradient heating design). FlexStation™ I. 1step assay with SpectraMax microplate readers. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. e. 5 Dual-luciferase reporter assay. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. View the Molecular Devices website. free tracer decreases, resulting in an. ® reader to the computer. B: Selection of Excitation Wavelength. Recertification is recommended every two years. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. TR-FRET assay. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 67. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit microplate reader. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. Methods Preparation of reagents and standards. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Maximum signal with lowest background. 63 1. System Diagrams and Dimensions. The cells were harvested on ice and. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Microplate Readers include the M3, M4, M5, and M5e models. However, when a specific probe is not available, or it causes off-target effects on the. 43 Max %CV 1. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 1** Version 7. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. Table of Contents. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Or scan up to 384 wells at any time to determine the best assay conditions. Pick the best speed, sensitivity, and price for your needs. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. No filter change with dual monochromator optics. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Wide range of applications. SpectraMax® iD5. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Detailed specifications of each model reader are reported in Section 7, “Specifications”. HTRF-compatible SpectraMax Multi-Mode Readers. The guide also covers the software features, the specifications, and the accessories of the systems. All associated StakMax® stackers. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. Category 2 and 3 microplate readers use gradient heating as well. SpectraMax® M5e. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 0 out of 5 $ 17,999 $ 16,995. 2. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 14 Average %CV 0. Sensitive fluorescent quantitation of DNA with the Quant-iT. Compact and tunable absorbance microplate readers DATA SHEET. PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. Lift up the lid over the chamber to insert or remove a cuvette. 1. 2. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Microbiota DNA was. Figure 1. Figure 1. Lower limits of detection for ATP determined using the ATPlite . The raw data from each individual experiment were normalized to vehicle-treated HK-2. The guide also covers the software features, the specifications, and the accessories of the systems. Retrieving Each. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. TR-FRET assay. keeping with best practices, reader performance should. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. View the Molecular Devices website. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. 05% Tween 20 using a Nunc ELISA plate washer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. E Models. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Page 28 Chapter 5: Read Modes and Read Types 1. 4 Max StDev 1. The acceptance. Temperature variation ± 0. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. At the end of this period, cells were exposed to FA (1 mM) for 1 h. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Microplate Capacity. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. These lab workhorses are used in drug discovery, research, bioassay validation, and. (Shanghai, China). SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Read More. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. Optical absorbance. Catalog Number: (89212-396) Supplier: Molecular Devices. Add to Cart. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. 00. Add to Cart. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). As the ratio of ADP:ATP increases, the proportion of bound tracer vs. When a feature or capability applies to only certain readers, this exception is noted. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Modethe introduction of the SPECTRAmax PLUS microplate spectrophotometer and its PathCheck feature, the ability to determine the pathlength in each well of a microplate and automatically normalize the absorbance value to a 1 cm pathlength. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Shop now. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Ferguson, Ph. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. com. 303 unable to cal dark current. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Microplate Readers include the M3, M4, M5, and M5e models. 96-1536. 6: bioNova científica, s. The SpectraMax M2 and M2e are versatile and reliable. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. Bioz Stars score: 86/100, based on 1 PubMed citations. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. The SpectraMax M5e Multi. M3-M5e_manual. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Table 1. The readings with excitation at 540 nm and emission at 600 nm represented the amount of phosphorylated IκB-α in the cells. The cuvette must be in place when you read the plate. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. es •. anisotropy was measured on SpectraMax M5 microplate reader with 485 nm excitation and 535 nm emission after 60 min incubation at room temperature. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. Keywords. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). Five preferred detection modes. 315 can't find zero order. Fluorescence intensity was detected by. We would like to show you a description here but the site won’t allow us. Linear, Orbital Slow, and Orbital Fast. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Microplate Readers include the M3, M4, M5, and M5e models. The spectrophotometric. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. 0 μL) instead of the sample solution was applied as control group, and. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Microplate Readers Biotek Epoch Microplate. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. Example: [Drawer]. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 9000-0756. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). Recommended Optics Wavelength (nm) Wavelength. Tunable wavelength selection / monochromator. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Levels of immunoreactivity were normalized to. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. Download PDF. l. Do not touch the clear surfaces of the cuvette. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Step 1. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. 3 Version 7. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. A: Microplate Preparation. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Here the delta mP was 336, and Z’ factor was 0. Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). AquaMax® 2000. for ValitaTiter Plus standards read on Molecular Devices readers. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. AquaMax® 4000. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. 6. 1 Version 7. Background fluorescence has been subtracted. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . book Page 7 Wednesday, July 7, 2010 2:09 PM. Add to Cart. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. The assay applies fluorescence polarization to quantify Fc-containing IgG. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. 58% for the detection module and 2. Molecular Devices Spectramax M5 Microplate Reader. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 7. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 1. Self Service Available. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 00 USD. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. 2. 6 0. 3 1. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. "With this complete portfolio of multi-mode configurations, we. FlexStation™ III. 6: bioNova científica, s. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. 0 out of 5 $ 16,499. edu) to be added as a trained user in iLab. flexibility of monochromator-based systems. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. 7 cells were co-transfected with 0. The cells were then treated to induce apoptosis. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Molecular Devices | Life Science Applications | Bioanalytical. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Western blotting analysis. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. SpectraMax® ABS. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Uncontrolled Copy for Training and Reference Purposes Only . The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. ATP standard curves read on SpectraMax microplate readers. E Models Only) 69. Make sure the microplate is flat against. The absorbance value of each reaction solution was measured at 405 nm by a Molecular Device SpectraMax M5 microplate reader (Sunnyvale, CA, USA). User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Ltd. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. 2. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. • Tel. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. 00 USD. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. Lift up the lid over the chamber to insert or remove a cuvette. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. 05 3. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). 96-384. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. M3-M5e_manual. 1. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Bookings are free, but you will need to use iLab to book. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. SpectraMax M5 Multi-Mode Microplate Reader. The assay window spanned four orders of magnitude. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. (The i3x and Paradigm readers require the. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. , Sunnyvale, CA) using the bottom well-scan mode where nine readings. Incubation. View the Molecular Devices website. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. 0 and 0. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Learn how you can utilize microplate readers to drive landmark discoveries. The spectrophotometric. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. SpectraMax M5e Multi-Mode Microplate Reader. Self Service Available. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. Molecular Devices Corporation. S. 95. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Figure 3. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. 1 is GxP edition only; Protocols for the ABS1 Validation Plate. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. 5. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The samples were boiled for 5 min with 1 ×. Molecular Devices SpectraMax M5 Multimode Microplate Reader. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. Use similar settings for other Molecular Devices readers with fluorescence detection mode. Molecular Devices. The Multiskan Sky microplate reader is an absorbance plate reader. begin reading microplates with seven mouse clicks. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. Plate Shaking. ZERO BIAS - scores, article reviews, protocol conditions and moreThe absorbance was measured at 450–650 nm using a SpectraMAX M5 microplate reader (Molecular Devices). 2. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. 4. Fifteen different automated tests are provided. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Microplate reader settings. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. Figure 4: Transcreener ADP. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader.